Open Access

BioSynthesis of Silver Nanoparticles Using Putrajiva Roxyburghii Wall Leaves extract and their Anti Bacterial Activities

Garima Kadiyan, garimakadiyan22@gmail.com
Department of Biotechnology, Lovley Professional University, Punjab, India.
Annubhawi Annu, garimakadiyan22@gmail.com
Department of Nanotechnology, Lovley Professional University, Punjab, India.
Amit Sachdeva garimakadiyan22@gmail.com
Department of Nanotechnology, Lovley Professional University, Punjab, India.



J. Environ. Nanotechnol., Volume 2, No 2 (June 2013) pp. 88-91

https://doi.org/10.13074/jent.2013.06.132008

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Abstract

The synthesis of nanoparticles from plant source has to be prove to be effective and alternate
method for the noble production of nanoparticles .A number of synthesis technique have been developed
including chemical method ,physical method and biosynthetic method. In this research synthesis of silver
nanoparticles using putranjiva roxyburghii wall leaves has been investigated .we successfully synthesized
uniformly dispersed silver nanoparticles with the size and shape in the range of 3 to 20nm with an average
size of 6 nm .The characterization of nanoparticles was done by using UV-VIS spectrophotometer and
fluorescent spectrometer.the resulting formation of silver nanoparticles are characterized by using
Transmission electron microscopy(TEM) .The TEM study shows the formation of silver nanoparticles .The
antibacterial activity of these nanoparticles was studied against E.coli and staphylococcus aurenus .The
bacterial property was analysed by measuring the growth curve of bacteria and 50ug/ml concentration of
silver nanoparticles was found to be effective antibacterial.

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