A Review of Green Synthesis of Silver Nanoparticles Using Plant Extracts: Mechanisms, Characterization, Comparative Analysis, Challenges, and Emerging Biomedical and Environmental Applications
J. Environ. Nanotechnol., Volume 15, No 3 (September 2026) pp. 463-476
Abstract
Silver nanoparticle (AgNP) production has attracted considerable interest due to its remarkable physicochemical properties and wide-ranging applications in catalysis, healthcare, and environmental science. Among various synthesis strategies, green synthesis using plant extracts has emerged as an efficient, economical, and environmentally friendly alternative to conventional chemical and physical methods. This approach uses abundant plant phytochemicals, including terpenoids, alkaloids, phenolics, and flavonoids, which act as natural reducing, stabilizing, and capping agents during nanoparticle formation. Recent advances have improved control over nanoparticle shape, size, and stability by optimizing parameters such as temperature, reaction time, pH, and extract concentration. This review critically synthesizes recent literature on the plant-mediated green synthesis of AgNPs by comparing different plant extracts, synthesis strategies, characterization techniques, and application outcomes. It also identifies current research challenges, knowledge gaps, and future directions for developing sustainable and scalable silver nanoparticle technologies. Comparative studies using different plant extracts reveal significant variations in reduction efficiency, particle size distribution, and application-specific performance. Green-synthesized AgNPs demonstrate strong antioxidant, antimicrobial, catalytic, and sensing capabilities, making them promising for biological applications, wastewater treatment, and pollutant degradation. Overall, the review highlights recent progress in plant-mediated AgNP synthesis, underlying green synthesis mechanisms, the essential role of phytochemicals, and the functional relevance of AgNPs, supported by selected reference studies and comparative assessments of synthesis outcomes.
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Ahmed, F., Abdou, M. and Gamal, A., Unraveling the mysteries of silver nanoparticles: synthesis, characterization, antimicrobial effects and uptake translocation in plant-a review, Planta, 260(1), 7 (2024).
https://doi.org/10.1007/s00425-024-04439-6
Amit, K. M., Yusuf, C. and Chand, U. B., Synthesis of metallic nanoparticles using plant extracts, Biotechnol. Adv., 31(2), 346-356 (2013).
https://doi.org/10.1016/j.biotechadv.2013.01.003
Andreescu, D., Reem, K., Mohamed, H. H., Jingyun, Y., Silvana, A., Nanoelectrochemistry Reveals Selective Interactions of Perfluoroalkyl Substances (PFASs) with Silver Nanoparticles, Angew Chem Int. Ed Engl., 61(42), e202209164 (2022).
https://doi.org/10.1002/anie.202209164
Asia, N., Faisal, A., Younas, F., Fentahun, W. G., Samir, I., Gamal, S. A. and Mohammed, B., Green-synthesized silver nanoparticles of Cinnamomum zeylanicum and their biological activities, Open Chem., 23(1), 20240128 (2025).
https://doi.org/10.1515/chem-2024-0128
Balaji, V., Sasikaran, R. R., Yuvakkumar, R., Arjun, B. K., Ravi, G. and Sambasivam, S., Green synthesis of silver nanoparticles from Citrus peel extracts for laboratory-scale electrochemical supercapacitor applications, Environ. Technol. Innov., 40, 10464 (2025).
https://doi.org/10.1016/j.eti.2025.104641
Bodaballa, A., Venu, R., Prasad, T. N. V. K. V., Alpha, R. M., Rao, S. K. and Srilatha, C., Synthesis and characterisation of neem leaf extract, 2, 3‐dehydrosalanol and quercetin dihydrate mediated silver nano particles for therapeutic applications, IET Nanobiotechnol., 11(4), 383–389 (2016).
https://doi.org/10.1049/iet-nbt.2016.0095
Brajesh, K., Smita, K., Angulo, Y., Debut, A. and Luis, C., China Rose/Hibiscus rosa-sinensis Pollen-Mediated Phytosynthesis of Silver Nanoparticles and Their Catalytic Activity, J. Compos. Sci., 6(11), 322 (2022).
https://doi.org/10.3390/jcs6110322
Chaudhari, R. K. and Shrivastav, P. S., Sustainable Synthesis of Silver Nanoparticles using Epipremnum aureum Leaf Extract: Application in Copper Ion Sensing and Antimicrobial Activity, Asian J. Chem., 36(9), 2120-2128 (2024).
https://doi.org/10.14233/ajchem.2024.32194
Chhangte, V., Samuel, L., Biswas, A., Selvaraj, M., Changmai, B. and Samuel, R. L., Green synthesis of silver nanoparticles using plant extracts and their antimicrobial activities: a review of recent literature, RSC Adv., 11(5), 2804–2837 (2021).
https://doi.org/10.1039/d0ra09941d
Chung, I. M., Park, L., Kim, S. H., Muthu, T. and Rajkumar, G., Plant-Mediated Synthesis of Silver Nanoparticles: Their Characteristic Properties and Therapeutic Applications, Nanoscale Res. Lett., 11(1), 40 (2016).
https://doi.org/10.1186/s11671-016-1257-4
Debasis, N., Ashe, S., Ranjan, R. P. and Nayak, B., Biosynthesis, characterisation and antimicrobial activity of silver nanoparticles using Hibiscus rosa-sinensis petals extracts, IET Nanobiotechnol., 9(5), 288–293 (2015).
https://doi.org/10.1049/iet-nbt.2014.0047
Dhir, R., Chauhan, S., Subham, P., Kumar, S., Sharma, P., Shidiki, A. and Kumar, G., Plant-mediated synthesis of silver nanoparticles: Unlocking their pharmacological potential—A comprehensive review, Front. Bioeng. Biotechnol., 11, 1324805 (2023).
https://doi.org/10.3389/fbioe.2023.1324805
Dutta, T., Ghosh, N. N., Das, M., Rajsekhar, A., Mandal, V. and Asoke, P. C., Green synthesis of antibacterial and antifungal silver nanoparticles using Citrus limetta peel extract: Experimental and theoretical studies, J. Environ. Chem. Eng., 8(4), 104019 (2020).
https://doi.org/10.1016/j.jece.2020.104019
Fatemeh, Z. and Ahmad, P. M., Innovative green and bio-based approaches for photosensitive nanoparticle synthesis: a review on methodologies, characterization, and applications, Micro Nano Syst. Lett., 13(1), 3 (2025).
https://doi.org/10.1186/s40486-025-00223-7
Hatice, D., Eker, F., Emir, A., Anna, W. M., Mikhael, B. and Karav, S., Silver Nanoparticles: A Comprehensive Review of Synthesis Methods and Chemical and Physical Properties, Nanomater., 14, 1527 (2024).
https://doi.org/10.3390/nano14181527
Kaviya, S., Santhanalakshmi, J., Viswanathan, B., Muthumary, J. and Srinivasan, K., Biosynthesis of silver nanoparticles using Citrus sinensis peel extract and its antibacterial activity, Spectrochimica Acta Part A, Mol. Biomol. Spectros., 79(3), 594–598 (2011).
https://doi.org/10.1016/j.saa.2011.03.040
Khan, K. and Javed, S., Silver nanoparticles synthesized using leaf extract of Azadirachta indica exhibit enhanced antimicrobial efficacy than the chemically synthesized nanoparticles: A comparative study, Sci. Prog., 104(2), 12159 (2021).
https://doi.org/10.1177/00368504211012159
Khwaja, S. S., Husen, A. and Rao, R. A. K., A review on biosynthesis of silver nanoparticles and their biocidal properties, J Nanobiotechnol., 16(1), 14 (2018).
https://doi.org/10.1186/s12951-018-0334-5
Kokila, T., Ramesh, P. S. and Geetha, D., Biosynthesis of silver nanoparticles from Cavendish banana peel extract and its antibacterial and free radical scavenging assay: a novel biological approach, Appl. Nanosci., 5(8), 911–920 (2015).
https://doi.org/10.1007/s13204-015-0401-2
Makarov, V. V., Love, A. J., Sinitsyna, O. V., Makarova, S. S., Yaminsky, I. V., Taliansky, M. E. and Kalinima, N. O., Green nanotechnologies: synthesis of metal nanoparticles using plants, Acta Nat., 6(1), 35-44 (2014).
https://doi.org/10.32607/20758251-2014-6-1-35-44
Mickky, B., Heba, E., Abbas, M., Ahmed, G. and Reham, S. E., Orange peel-mediated synthesis of silver nanoparticles with antioxidant and antitumor activities, BMC Biotechnol., 24(1), 66 (2024).
https://doi.org/10.1186/s12896-024-00892-z
Moinuzzaman, Abu, M. M. H. J., Helal, U., Rahman, S., Abdul, M. and Abdullah, A. M., Biogenic Synthesis of Silver Nanoparticles using Azadirachta indica (Neem) Leaf Extract to Achieve Inhibitory Action Against Antibiotic-Resistant Bacteria, Anti-Infective Agents., 19(5), 90464 (2021).
https://doi.org/10.2174/2211352519666210614115340
Moza, K., Sarma, M. P., Zaki, M. S. and Neonjyoti, B., Green Synthesis of Silver Nanoparticles Using Citrus sinensis Peel Extract, Int. J. Environ. Sci., 11(4), 2229-7359 (2025).
Munnaluri, G., Nivedita, S., Goud, M. N., Boopalan, K. and Kavitha, T., Synthesis of Silver Nanoparticles using Cinnamomum zeylanicum Bark Extract and its Antioxidant Activity, Nanosci. Nanotechnol. Asia., 5(1), 2-7 (2015).
https://doi.org/10.2174/221068120501150728103209
Nadiyah, M. A. and Md., Mahadi, H., Plant-based green synthesis of silver nanoparticles and its effective role in abiotic stress tolerance in crop plants, Saudi J. Bio. Sci., 28(10), 5631-5639 (2021).
https://doi.org/10.1016/j.sjbs.2021.05.081
Niluxsshun, C. D. M., Koneswaran, M. and Umaramani, M., Green Synthesis of Silver Nanoparticles from the Extracts of Fruit Peel of Citrus tangerina, Citrus sinensis, and Citrus limon for Antibacterial Activities, Bioinorg. Chem. Appl., 2021, 1-8 (2021).
https://doi.org/10.1155/2021/6695734
Noor, M., Tamara, D., Marco, F. and Ulrich, S., Engineering of the E. coli Outer Membrane Protein FhuA to overcome the HydrophobicMismatch in Thick Polymeric Membranes, J. Nanobiotechnol., 9, 8 (2011).
https://doi.org/10.1186/1477-3155-9-8
Ogundare, S. A., Adesetan, T. O., Muungani, G., Moodley, V., Amaku, J. F., Atewolara-Odule, O. C., Yussuf, S. T., Sanyaolu, N. O., Ibikunle, A. A., Balogun, M. S. and Van Zyl, W., Catalytic degradation of methylene blue dye and antibacterial activity of biosynthesized silver nanoparticles using Peltophorum pterocarpum (DC.) leaves, Environ. Sci. Adv., 2(2), 247-256 (2023).
https://doi.org/10.1039/D2VA00164K
Pal, S., Tak, Y. and Song, J. M., Does the antibacterial activity of silver nanoparticles depend on the shape of the nanoparticle? A study of the Gram-negative bacterium Escherichia coli, Appl. Environ. Microbio., 73(6), 1712-1720 (2007).
https://doi.org/10.1128/AEM.02218-06
Patterson, A. L., The Scherrer Formula for X-Ray Particle Size Determination, Phy. Rev., 56(10), 978–982 (1939).
https://doi.org/10.1103/PhysRev.56.978
Pragyan, R., Das, B., Abhipsa, M. and Sujata, M., Green synthesis of silver nanoparticles using Azadirachta indica leaf extract and its antimicrobial study, Appl. Nanosci.,7(8), 843–850 (2017).
https://doi.org/10.1007/s13204-017-0621-8
Priyanga, R., Pigera, S., Ga, P. S., Priyadharshini, G., Godwin, C. R. and Prasad, K., Medicinal properties of ‘true’ cinnamon (Cinnamomum zeylanicum): a systematic review, BMC Complement Altern Med., 13(1), 275 (2013).
https://doi.org/10.1186/1472-6882-13-275
Raghupathi, C. H., Vijaya, K. A., Sujatha, S., Shashi, K. M., Shirisha, A., Krishnaiah, N. and Pradeep, K. A., Green synthesis of silver nanoparticles using Azadirachta indica and Ocimum sanctum and assessment of its antimicrobial activity against isolates and pure culture of Escherichia coli, Pharma Innov. J., 11(4), 320-330 (2022).
Randa, M. D., Bio fabrication of Nano silver From Punica granatum Peel Extract and Their Anticoagulant Applications, Int. J. Food Sci., 2024(1), 6623228 (2024).
https://doi.org/10.1155/2024/6623228
Reyam, F. S. and Ayad, M., G., Biosynthesis and characterization of silver nanoparticles using Cinnamomum zeylanicum extract and a study of antibacterial effect against multi-drug resistance Gram-negative bacteria, Biomed., 41(2), 249-255 (2021).
https://doi.org/10.51248/.v41i2.791
Sanjeev, K., Shashi, S., Vinay, P., Rajaput, T. N., Deepak, P., Sharma, B., Sharma, D., Kar, N. R., Suman, A. and Odisha, Green Synthesis of Silver Nanoparticles from Combined Leaf Extracts, J. Cardiovascular Disease Res., 14(9), 1036-1053 (2023).
https://doi.org/10.48047/jcdr.2023.14.09.107
Sathiya, R. P., Geetha, D. and Ramesh, P. S., Antioxidant activity of chemically synthesized AgNPs and biosynthesized Pongamia pinnata leaf extract mediated AgNPs - A comparative study, Ecotoxicol Environ. Saf., 134, 308-318 (2016).
https://doi.org/10.1016/j.ecoenv.2015.07.037
Savita, P., Ajaya, K. S., Bhawana, J., Gautam, S. T., Green Synthesis of Silver Nanoparticles by Mint Leaves: A Suitable Catalyst for Oxidation of Metronidazole, Asian J. Chem., 29(11), 2409-2414 (2017).
https://doi.org/10.14233/ajchem.2017.20704
Shimaa, H., Gaber, G. A., Ragab, M. S., Mohamed, A. R., Anter, M. and Lamiaa, Z. M., A Comprehensive Review of Silver Nanoparticles (AgNPs): Synthesis Strategies, Toxicity Concerns, Biomedical Applications, AI-Driven Advancements, Challenges, and Future Perspectives, Arab. J. Sci. Eng., 51(11), 13667-13714 (2025).
https://doi.org/10.1007/s13369-025-10612-0
Supritha, C. T., Green Synthesis and Characterization of Silver Nanoparticles using Cinnamomum zeylanicum Bark Extract, Int. J.Curr. Microbiol. App.Sci.,10, 444-451 (2021).
https://doi.org/10.20546/ijcmas.2021.1001.055
Thomas, S., Gonsalves, R. A., Jose, J., Zyoud, S. H., Prasad, A. R. and Garvasis, J., Plant-based synthesis, characterization approaches, applications and toxicity of silver nanoparticles: A comprehensive review, J. Biotechnol., 394, 135–149 (2024).
https://doi.org/10.1016/j.jbiotec.2024.08.009
Tijjani, M., Misni, N., Nur, I. R., Daskum, A. M. and Ngah, U. Z., A Review on Plants and Microorganisms Mediated Synthesis of Silver Nanoparticles, Role of Plants Metabolites and Applications, Int. J. Environ. Res., 19(2), 674 (2022).
https://doi.org/10.3390/ijerph19020674
Varadharajan, S. and Pandian, P., Antioxidant, antimicrobial, and potential biomedical applications of green-synthesized silver nanoparticles using Aloe barbadensis root, J. Environ. Nanotechnol., 14(1), 97–103 (2025).
https://doi.org/10.13074/jent.2025.03.2511226
Vanaja, M. and Annadurai, G., Coleus aromaticus leaf extract mediated synthesis of silver nanoparticles and its bactericidal activity, Appl. Nanosci., 3(3), 217-223 (2013).
https://doi.org/10.1007/s13204-012-0121-9
Vidyasagar, R., Patel, R. R., Singh, S. K. and Singh, M., Green synthesis of silver nanoparticles: methods, biological applications, delivery and toxicity, Mater. Adv., 4(8), 1831–1849 (2023).
https://doi.org/10.1039/D2MA01105K
Villagrán, Z., Luis, M. A. E., Carlos, A. V. C., Jorge, M. S.J., Jose, M. R. G., Vigo, E. F. A., Ernesto, R, L., Noe, R. B., Ivan, B. L. and Fernando, M. E., Plant-Based Extracts as Reducing, Capping, and Stabilizing Agents for the Green Synthesis of Inorganic Nanoparticles, Resources., 13(6), 70 (2024).
https://doi.org/10.3390/resources13060070
Wirwis, A. and Zygmunt, S., Green Synthesis of Silver Nanoparticles: Optimizing Green Tea Leaf Extraction for Enhanced Physicochemical Properties, ACS Omega, 8(33), 30532-30549 (2023).
https://doi.org/10.1021/acsomega.3c03775
Yasmeen E. M., Elsebaei, F. and Mohammed, E. M., Bio inspired one pot synthesis of luminescent silver nanoparticles and its significant utility as a fluorescence nano sensor for analysis of two adjunctive COVID 19 drugs, BMC Chem., 18(1), 227 (2024).
