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Extraction, Isolation, Characterization and DNA Binding Study of Carbazole Alkaloids from Murraya Koenigii (Curry Leaf )

V. Mohanraj, Department of chemistry, Sri Ramakrishna mission Vidyalaya College of Arts and Science, Coimbatore, TN, India. M. Dhandapani, Department of chemistry, Sri Ramakrishna mission Vidyalaya College of Arts and Science, Coimbatore, TN, India. G. Amirthaganesan, profga@yahoo.co.in
Department of chemistry, Sri Ramakrishna mission Vidyalaya College of Arts and Science, Coimbatore, TN, India. M. Sekar Department of chemistry, Sri Ramakrishna mission Vidyalaya College of Arts and Science, Coimbatore, TN, India.

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Abstract

Murraya Koenigii is a medium sized tree belonging to the family rutaceae. The present study aimed to investigate the phytochemical composition of the stem bark of murraya koenigii. The powdered plant material was extracted by maceration with acetone, and the acetone extract was fractionated with petroleum ether and subjected to column chromatography. The structure were established by interpretation of their UV-VIS , 1 H, 13 C, DEPT, 1 H-1 H COSY, 13 C-1 H COSY NMR Spectroscopy and Mass spectrometry. The investigations led to isolation of two constituents were identified, such as Girinimbilol compound can be isolated by petroleum ether, another one compound cannot be isolated by column chromatography. The isolated compound was subjected to DNA binding study with Calf thymus DNA. The binding affinity value is 1.324 x 10 5 m -1 . DNA binding study of the compound isolated from M.Koenigii is the first time reported method. The anti cancer study of the compound in the HeLa cell line with MTT assay method to IC 50 value is 178 μM.

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