Effect of Burkholderia sp. on Root Nodulation in Black Gram Grown in Cadmium-Contaminated Soil Supplemented with Lignite Humic Acid and Seaweed Biochar
J. Environ. Nanotechnol., Volume 13, No 4 (2024) pp. 130-148
Abstract
A non-rhizobial, root-nodulating endophytic bacterium, Burkholderia multivorans strain Strulens, was isolated from black gram root nodules. Under cadmium (Cd) stress, this bacterium synthesized indole-3-acetic acid (IAA), produced exopolysaccharides, and solubilized phosphate. When black gram plants were treated with biochar (2% w/w), humic acid (2% w/w), and Burkholderia sp., they exhibited increased nodule formation and higher leghemoglobin content, even at varying Cd concentrations. Combining humic acid and biochar with Burkholderia sp. also reduced Cd translocation from the roots to the shoots, resulting in a translocation factor of 0.17. These findings suggest that incorporating biochar and humic acid into Cd-affected soils, along with Burkholderia sp. inoculation, can improve legume growth and reduce Cd uptake in contaminated soils.
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Reference
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