Chavibetol Loaded Zinc Oxide Nanoparticles (CB-ZnONPs) for Activity-Enhanced Anticancer Applications against Human Lung (A549)
J. Environ. Nanotechnol., Volume 13, No 4 (2024) pp. 304-315
Abstract
Chavibetol loaded Zinc oxide nanoparticles have demonstrated exceptional potential as anticancer agents when used together. Collectively, it possesses the capacity to serve as a substitute or addition to conventional anticancer medications. The current study focuses on examining the deliberate synthesis of biocompatible Zinc oxide nanoparticles (CB-ZnO-NPs) that are infused with chavibetol. We also assessed these nanoparticles for their ability to load and release chavibetol as a prodrug. Furthermore, the loaded particles were assessed for their ability to inhibit cell growth and induce programmed cell death in Human Lung (A549). The structure and morphological properties of the produced materials were analyzed by scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction analysis, dynamic light scattering analysis, and zeta potential analysis. Dynamic light scattering (DLS) and scanning electron microscopy (SEM) examination verifies that the synthesized CB-ZnONPs have a size of around 50-60 nm. Furthermore, the analysis confirms that the nanoparticles possess a highly crystalline structure and exhibit a rod shape. The FTIR measurement confirms the conjugation of chavibetol molecules with zinc oxide nanoparticles. The X-ray diffraction patterns reveal that the zinc oxide nanoparticles possess a spinel structure and exhibit a high level of purity. Chavibetol-loaded nanoparticles exhibit a regulated release pattern in accordance with different pH levels. We observed a notable suppression of cell growth in the group that received treatment, followed by the occurrence of programmed cell death as indicated by the study of fluorescence microscopy using AO/EtBr. The Synthesized material also showed that upregulation of apoptotic protein and down regulating the antiapoptotic protein. The results of our study clearly show that chavibetol loaded zinc oxide nanoparticles may effectively transport the desired medication and induce programmed cell death in specific human lung cancer cells. This has the potential to initiate a new field in polymer surface chemistry, focusing on the creation of drug delivery systems for cancer therapies using metal oxide materials.
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