Open Access

Enhancing Shelf Lifestorage Stability of Anaerobically Fermented Garbage Enzyme by Concentrating through Membrane Separation

P. Mala, Department of Biotechnology, Periyar Maniammai Institute of Science & Technology, Vallam, Thanjavur, TN, India A. Ashokkumar, ashokkumar@pmu.edu
Department of Biotechnology, Periyar Maniammai Institute of Science & Technology, Vallam, Thanjavur, TN, India C. Arun , Department of Biotechnology, Periyar Maniammai Institute of Science & Technology, Vallam, Thanjavur, TN, India K. Geetha Department of Biotechnology, Periyar Maniammai Institute of Science & Technology, Vallam, Thanjavur, TN, India

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Abstract

Enzyme solutions derived from garbage have many applications in the surfactant industry, industrial and municipal sludge stabilization, Bio-fertilizer, bio-herbicide & bio-pesticide production, etc. Once the produced solution is taken into an aerobic environment, it induces microbial growth which may in turn produce desirable and undesirable biochemicals. To control the activation of biochemicals present in garbage-fermented solution by removing water content using a membrane separation process was investigated. Fabricated Polyvinylidene Fluoride (PVDF) membrane separation set up with 0.45 µm pores produces a bifurcated yield of concentrate and water, whichis a similar process as a water purification unit in the home. As the water part is reduced by membrane separation from the enzyme solution the reaction with oxygen is controlled in a concentrated part which in turn reduces the hydrolysis reaction. The inactive condition was investigated by altering the pH of the system, which also reduced BOD and COD the main impact of increasing the stability of the enzyme.The experiment investigation is executed, discussed, and reported for the transport process parameters on the membrane process and control investigation by the parameter pH. BOD and COD. This is a trial attempt to check the perception of how far the membrane separation process works on concentrating the garbage-produced enzyme solution as well as reducing the rate of degradation while in contact with air. Also, the activity of protease, lipase, and amylase was increased in the concentrated garbage enzyme.

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Reference

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Arun, C., Sivashanmugam, P., Solubilization of waste activated sludge using a garbage enzyme produced from different pre-consumer organic waste, RSC Adv. 5(63), 51421–51427 (2015a).

https://doi.org/10.1039/C5RA07959D

Arun, C., Sivashanmugam, P., Study on optimization of process parameters for enhancing the multi-hydrolytic enzyme activity in garbage enzyme produced from preconsumer organic waste, Bioresour. Technol. 226, 200–210 (2017).

https://doi.org/10.1016/j.biortech.2016.12.029

Arun, C., Sivashanmugam, P., Investigation of biocatalytic potential of garbage enzyme and its influence on stabilization of industrial waste activated sludge, Process Saf. Environ. Prot. 94, 471–478 (2015b).

https://doi.org/10.1016/j.psep.2014.10.008

Bhattacharjee, C., Saxena, V. K., Dutta, S., Fruit juice processing using membrane technology: A review, Innov. Food Sci. Emerg. Technol. 43, 136–153 (2017).

https://doi.org/10.1016/j.ifset.2017.08.002

Katibi, K. K., Mohd Nor, M. Z., Yunos, K. F. M., Jaafar, J., Show, P. L., Strategies to Enhance the Membrane-Based Processing Performance for Fruit Juice Production: A Review, Membranes (Basel). 13(7), 679 (2023).

https://doi.org/10.3390/membranes13070679

Kowalczewski, P. Ł., Olejnik, A., Rybicka, I., Zielińska-Dawidziak, M., Białas, W., Lewandowicz, G., Membrane Filtration-Assisted Enzymatic Hydrolysis Affects the Biological Activity of Potato Juice, Molecules 26(4), 852 (2021).

https://doi.org/10.3390/molecules26040852

Nongonierma, A. B., FitzGerald, R. J., Unlocking the biological potential of proteins from edible insects through enzymatic hydrolysis: A review, Innov. Food Sci. Emerg. Technol. 43, 239–252 (2017).

https://doi.org/10.1016/j.ifset.2017.08.014

Padhan, B., Ray, M., Patel, M., Patel, R., Production and Bioconversion Efficiency of Enzyme Membrane Bioreactors in the Synthesis of Valuable Products, Membranes (Basel). 13(7), 673 (2023).

https://doi.org/10.3390/membranes13070673

Pimentel, G. A., Almeida, P., Hantson, A.-L., Rapaport, A., Vande Wouwer, A., Experimental validation of a simple dynamic model of a laboratory scale recirculating aquaculture system fitted with a submerged membrane bioreactor, Biochem. Eng. J. 122, 1–12 (2017).

https://doi.org/10.1016/j.bej.2017.02.005

Prazeres, D. M. F., Cabral, J. M. S., Enzymatic membrane bioreactors and their applications, Enzyme Microb. Technol. 16(9), 738–750 (1994).

https://doi.org/10.1016/0141-0229(94)90030-2

Sambaraju, S., Sree Lakshmi, V., Eco-friendly treatment of dairy wastewater using garbage enzyme, Mater. Today Proc. 33, 650–653 (2020).

https://doi.org/10.1016/j.matpr.2020.05.719

Schmidt, M., Prager, A., Schönherr, N., Gläser, R., Schulze, A., Reagent-Free Immobilization of Industrial Lipases to Develop Lipolytic Membranes with Self-Cleaning Surfaces, Membranes (Basel). 12(6), 599 (2022).

https://doi.org/10.3390/membranes12060599

Servent, A., Abreu, F. A. P., Dhuique-Mayer, C., Belleville, M.-P., Dornier, M., Concentration and purification by crossflow microfiltration with diafiltration of carotenoids from a by-product of cashew apple juice processing, Innov. Food Sci. Emerg. Technol. 66, 102519 (2020).

https://doi.org/10.1016/j.ifset.2020.102519

Sitanggang, A. B., Drews, A., Kraume, M., Enzymatic membrane reactors: Designs, applications, limitations and outlook, Chem. Eng. Process. - Process Intensif. 180, 108729 (2022).

https://doi.org/10.1016/j.cep.2021.108729